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dapi nuclear staining  (Santa Cruz Biotechnology)


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    Structured Review

    Santa Cruz Biotechnology dapi nuclear staining
    Dapi Nuclear Staining, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 1459 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/staining+with+dapi/DAPI/pm42869916-314-14-20
    Average 96 stars, based on 1459 article reviews
    dapi nuclear staining - by Bioz Stars, 2026-10
    96/100 stars

    Images

    Related Articles

    Immunofluorescence:

    Article Title: Interaction of OIP5-AS1 with MEF2C mRNA promotes myogenic gene expression
    Article Snippet: .. Antibody B-5,#B0620 (Santa Cruz Biotechnology) was used to detect MYH by immunofluorescence; staining with DAPI (4′,6-diamidino-2-phenylindole) was used to identify nuclei. ..

    Article Title: Are alsin and spartin novel interaction partners?
    Article Snippet: 0006-291X/$ see front matter 2012 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbrc.2012.08.103 ⇑ Corresponding author.. Address: Department of Mo Neurodegeneration Research Laboratory (NDAL), B Turkey.. Fax: +90 212 3597298.

    Staining:

    Article Title: Interaction of OIP5-AS1 with MEF2C mRNA promotes myogenic gene expression
    Article Snippet: .. Antibody B-5,#B0620 (Santa Cruz Biotechnology) was used to detect MYH by immunofluorescence; staining with DAPI (4′,6-diamidino-2-phenylindole) was used to identify nuclei. ..

    Article Title: Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human
    Article Snippet: Hepa1-6 cells, HepG2 cells, rat primary hepatocytes, rat BM-MSCs, and rat BM-MSC-derived hepatocyte-like cells were cultured on glass coverslips for 24 h. After the cells were fixed, they were incubated with a primary antibody against ALB (1:100, cat no. ab8940; Abcam, Cambridge, MA, USA) and a FITC-conjugated secondary anti-sheep IgG (cat no. ab6743; Abcam), according to the manufacturer's instructions. .. After nuclear staining with DAPI (Santa Cruz Biotechnology, Dallas, TX, USA) slides were observed with a fluorescence microscope (Leica, Germany). ..

    Article Title: Angiotensin 1–7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression
    Article Snippet: The cells were incubated with rabbit anti-TLR4 (ab22048; 1:200; Abcam, Cambridge, UK) and anti-NF-κB p65 (8242; 1:200; Cell Signaling Technology, Inc., Danvers, MA, USA) or control rabbit immunoglobulin G (IgG; ab6730; 1:200; Abcam, Cambridge, UK) at 4°C overnight. .. Cells were washed and incubated with fluorescein isothiocyanate-conjugated goat anti-rabbit IgG (bs-0295M-FITC; 1:100; BIOSS, Beijing, China) for 40 min at 37°C followed by staining with DAPI (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). ..

    Article Title: MiR-4270 acts as a tumor suppressor by directly targeting Bcl-xL in human osteosarcoma cells
    Article Snippet: DNA content after staining of trypsinized cells was performed by nuclear staining with propidium iodide (Sigma-Aldrich, Saint-Louis, MO, USA) using a Cytoflex S Flow Cytometer (Beckman Coulter France SAS, Paris, France) as previously described ( ). .. Morphological characterization of detached cells and trypsinized cells by nuclear staining with DAPI (4’,6-diamidino-2-phenylindole, dilactate) (Santa Cruz Biotechnology, Dallas, TX, USA) was carried out as previously described ( ). .. Protein levels of both detached and adherent cells were analyzed by immunoblotting with PARP (9542), Bcl-xL (2764) and Bak (3814) antibodies from Cell Signaling Technology (Ozyme, Saint-Quentin-en-Yvelines, France), with Mcl-1 (S-19), GAPDH (FL-335) and β-Tubulin (D-10) antibodies from Santa Cruz Biotechnology (Dallas, TX, USA), and with the Bcl-2 (M0887) antibody from DAKO (Agilent, Santa Clara, CA, USA) as previously described ( , ).

    Article Title: Are alsin and spartin novel interaction partners?
    Article Snippet: 0006-291X/$ see front matter 2012 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbrc.2012.08.103 ⇑ Corresponding author.. Address: Department of Mo Neurodegeneration Research Laboratory (NDAL), B Turkey.. Fax: +90 212 3597298.

    Article Title: MiR-4270 acts as a tumor suppressor by directly targeting Bcl-xL in human osteosarcoma cells.
    Article Snippet: DNA content after staining of trypsinized cells was performed by nuclear staining with propidium iodide (Sigma-Aldrich, SaintLouis, MO, USA) using a Cytoflex S Flow Cytometer (Beckman Coulter France SAS, Paris, France) as previously described (26). .. Morphological characterization of detached cells and trypsinized cells by nuclear staining with DAPI (4’,6-diamidino-2phenylindole, dilactate) (Santa Cruz Biotechnology, Dallas, TX, USA) was carried out as previously described (26). .. Protein levels of both detached and adherent cells were analyzed by immunoblotting with PARP (9542), Bcl-xL (2764) and Bak (3814) antibodies from Cell Signaling Technology (Ozyme, SaintQuentin-en-Yvelines, France), with Mcl-1 (S-19), GAPDH (FL335) and b-Tubulin (D-10) antibodies from Santa Cruz Biotechnology (Dallas, TX, USA), and with the Bcl-2 (M0887) antibody from DAKO (Agilent, Santa Clara, CA, USA) as previously described (26, 27).

    Article Title: Tumor Suppressive Role of miR-342-5p and miR-491-5p in Human Osteosarcoma Cells
    Article Snippet: Data were analyzed by Cytexpert ® acquisition software (Beckman Coulter France SAS, Paris, France). .. Morphological characterization of apoptotic cells by nuclear staining with DAPI (sc-24941, Santa Cruz Biotechnology, Dallas, TX, USA) was performed after cyto-centrifugation as previously described [ ]. .. Cell confluence and nuclear staining were monitored using a fluorescence microscope (Nikon Eclipse Ti; Nikon Instruments Inc., Melville, NY, USA).

    Article Title: Recombinant Adenosine Deaminase Ameliorates Inflammation, Vascular Disease, and Fibrosis in Preclinical Models of Systemic Sclerosis.
    Article Snippet: through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1002/ART.41259 This article is protected by copyright.. All rights reserved DR. HONGLIN ZHU (Orcid ID : 0000-0001-8724-2835) DR. ALINA SOARE (Orcid ID : 0000-0002-3881-2325) PROF. GEORG SCHETT (Orcid ID : 0000-0001-8740-9615) DR. JÖRG H.W.

    Fluorescence:

    Article Title: Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human
    Article Snippet: Hepa1-6 cells, HepG2 cells, rat primary hepatocytes, rat BM-MSCs, and rat BM-MSC-derived hepatocyte-like cells were cultured on glass coverslips for 24 h. After the cells were fixed, they were incubated with a primary antibody against ALB (1:100, cat no. ab8940; Abcam, Cambridge, MA, USA) and a FITC-conjugated secondary anti-sheep IgG (cat no. ab6743; Abcam), according to the manufacturer's instructions. .. After nuclear staining with DAPI (Santa Cruz Biotechnology, Dallas, TX, USA) slides were observed with a fluorescence microscope (Leica, Germany). ..

    Article Title: Are alsin and spartin novel interaction partners?
    Article Snippet: 0006-291X/$ see front matter 2012 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbrc.2012.08.103 ⇑ Corresponding author.. Address: Department of Mo Neurodegeneration Research Laboratory (NDAL), B Turkey.. Fax: +90 212 3597298.

    Microscopy:

    Article Title: Construction of a general albumin promoter reporter system for real-time monitoring of the differentiation status of functional hepatocytes from stem cells in mouse, rat and human
    Article Snippet: Hepa1-6 cells, HepG2 cells, rat primary hepatocytes, rat BM-MSCs, and rat BM-MSC-derived hepatocyte-like cells were cultured on glass coverslips for 24 h. After the cells were fixed, they were incubated with a primary antibody against ALB (1:100, cat no. ab8940; Abcam, Cambridge, MA, USA) and a FITC-conjugated secondary anti-sheep IgG (cat no. ab6743; Abcam), according to the manufacturer's instructions. .. After nuclear staining with DAPI (Santa Cruz Biotechnology, Dallas, TX, USA) slides were observed with a fluorescence microscope (Leica, Germany). ..

    Article Title: Are alsin and spartin novel interaction partners?
    Article Snippet: 0006-291X/$ see front matter 2012 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbrc.2012.08.103 ⇑ Corresponding author.. Address: Department of Mo Neurodegeneration Research Laboratory (NDAL), B Turkey.. Fax: +90 212 3597298.

    Incubation:

    Article Title: Angiotensin 1–7 ameliorates caerulein-induced inflammation in pancreatic acinar cells by downregulating Toll-like receptor 4/nuclear factor-κB expression
    Article Snippet: The cells were incubated with rabbit anti-TLR4 (ab22048; 1:200; Abcam, Cambridge, UK) and anti-NF-κB p65 (8242; 1:200; Cell Signaling Technology, Inc., Danvers, MA, USA) or control rabbit immunoglobulin G (IgG; ab6730; 1:200; Abcam, Cambridge, UK) at 4°C overnight. .. Cells were washed and incubated with fluorescein isothiocyanate-conjugated goat anti-rabbit IgG (bs-0295M-FITC; 1:100; BIOSS, Beijing, China) for 40 min at 37°C followed by staining with DAPI (Santa Cruz Biotechnology, Inc., Dallas, TX, USA). ..

    Imaging:

    Article Title: Are alsin and spartin novel interaction partners?
    Article Snippet: 0006-291X/$ see front matter 2012 Elsevier Inc. A http://dx.doi.org/10.1016/j.bbrc.2012.08.103 ⇑ Corresponding author.. Address: Department of Mo Neurodegeneration Research Laboratory (NDAL), B Turkey.. Fax: +90 212 3597298.

    Marker:

    Article Title: Recombinant Adenosine Deaminase Ameliorates Inflammation, Vascular Disease, and Fibrosis in Preclinical Models of Systemic Sclerosis.
    Article Snippet: through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1002/ART.41259 This article is protected by copyright.. All rights reserved DR. HONGLIN ZHU (Orcid ID : 0000-0001-8724-2835) DR. ALINA SOARE (Orcid ID : 0000-0002-3881-2325) PROF. GEORG SCHETT (Orcid ID : 0000-0001-8740-9615) DR. JÖRG H.W.

    Bioprocessing:

    Article Title: Recombinant Adenosine Deaminase Ameliorates Inflammation, Vascular Disease, and Fibrosis in Preclinical Models of Systemic Sclerosis.
    Article Snippet: through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record.. Please cite this article as doi: 10.1002/ART.41259 This article is protected by copyright.. All rights reserved DR. HONGLIN ZHU (Orcid ID : 0000-0001-8724-2835) DR. ALINA SOARE (Orcid ID : 0000-0002-3881-2325) PROF. GEORG SCHETT (Orcid ID : 0000-0001-8740-9615) DR. JÖRG H.W.



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    Image Search Results


    LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Journal: International Journal of Nanomedicine

    Article Title: Lactoferrin-Modified Ultra-Sustained Release Propofol Nanoparticles for Multi-Pathway Therapy of Depression via Intraperitoneal Injection

    doi: 10.2147/IJN.S645818

    Figure Lengend Snippet: LF-PVA@Pro NPs alleviate CRS-induced neuropathological damage and restore neuronal activity and neurotransmitter-related signaling in the VTA and NAc. ( A ) Representative H&E staining images of the ventral tegmental area and nucleus accumbens from the control, CRS, free-propofol, PVA@Pro NPs and LF-PVA@Pro NPs groups. Boxed regions are shown at higher magnification. ( B ) Quantification of histopathological injury scores in the VTA and NAc. ( C ) Representative Nissl-staining images of the VTA and NAc in the indicated groups, with enlarged views of the selected regions. ( D) Quantification of morphologically normal Nissl-positive neurons in the VTA and NAc. ( E ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the VTA. Nuclei were counterstained with DAPI. ( F ) Quantification of c-Fos and GABA fluorescence intensities in the VTA. ( G ) Quantification of c-Fos and DAT fluorescence intensities in the VTA. ( H ) Representative immunofluorescence images of c-Fos&GABA and c-Fos&DAT staining in the NAc. ( I ) Quantification of c-Fos and GABA fluorescence intensities in the NAc. ( J ) Quantification of c-Fos and DAT fluorescence intensities in the NAc. CRS exposure caused marked histopathological injury, loss of Nissl-positive neurons, and reductions in c-Fos-, GABA-, and DAT-associated fluorescence signals in the VTA and NAc. LF-PVA@Pro NPs treatment attenuated tissue injury and produced greater recovery of neuronal integrity and functional marker expression than free propofol. Data are presented as the mean ± SD (n=3). *p <0.05, p <0.01, ***p <0.001 , and ****p <0.0001 ; ns, not significant.

    Article Snippet: The sections were subsequently counterstained with DAPI staining solution (Boster Biological Technology Co., Ltd.) for 15 min.

    Techniques: Activity Assay, Staining, Control, Immunofluorescence, Fluorescence, Tissue, Produced, Functional Assay, Marker, Expressing